Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor
نویسندگان
چکیده
منابع مشابه
Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor
Introduction Cell based therapy requires great numbers of cells in a functional state permitting their in vivo implantation for the restoration of tissue homeostasis. Three main parameters are believed to be essential for such a purpose: an appropriate cell population, a suitable scaffold and appropriate physical / biochemical factors enabling proper expansion and in vitro cell differentiation....
متن کاملMaximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system.
Bioreactor systems have been developed as alternatives to standard culture flasks due to their homogeneous nature, easiness of monitoring and increased cell production. Here we investigated the in vitro expansion of bone marrow (BM) mesenchymal stem cells (MSC) in spinner flasks, using gelatin microcarriers (Cultispher S) to support cell adhesion and proliferation. MSC expansion was performed u...
متن کاملMicrocarrier Culture for Efficient Expansion and Osteogenic Differentiation of Human Fetal Mesenchymal Stem Cells
Stirred microcarrier (MC) culture has been suggested as the method of choice for supplying large volumes of mesenchymal stem cells (MSCs) for bone tissue engineering. In this study, we show that in addition to the improvement in cell expansion capacity, MSCs propagated and harvested from MC culture also demonstrate higher osteogenic potency when differentiated in vivo or in vitro in three-dimen...
متن کاملEx vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow
Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then different...
متن کاملExpansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions
Materials and methods Preliminary tests in disposable-spinners (100 mL culture volume, Corning) were used to determine two suitable media and MC-types for serum reduced expansions (< 10%) of human adipose tissue-derived stem cells (hADSCs; passage 2, Lonza). Using such optimized media-MC-combinations, hADSCs expanded 30 to 40fold, which compares well with expansion rates in planar culture. Base...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2011
ISSN: 1753-6561
DOI: 10.1186/1753-6561-5-s8-p55